Human IL-25/IL-17E protein (Recombinant) (C-hFc&His) (STJP002998)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human IL-25/IL-17E Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr33-Gly177) of Human IL-25/IL-17E (Accession #NP_073626.1) fused with a His tag at the N-terminus.
General Information
| Short Description | Recombinant-Human IL-25/IL-17E-C-hFc&His protein was developed in hek293 cells using the region Tyr33-Gly177. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 5.46-21.84ng/mL, corresponding to a specific activity of 4.58×104~1.83×105 units/mg. |
| Determination Method | <0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IL25 |
| Gene ID | 64806 |
| Uniprot ID | IL25_HUMAN |
| Immunogen | Recombinant Human IL-25/IL-17E Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Tyr33-Gly177) of Human IL-25/IL-17E (Accession #NP_073626.1) fused with a His tag at the N-terminus. |
| Immunogen Region | Tyr33-Gly177 |
| Immunogen Sequence | HPLMVSDRMDLDTLARSRQL EKRMSEEMRLLEMEFEERKR AAEQRQKAENFWTGDTSSDQ LVLGKKDMGWPFQADGQEG |
Additional Info
| Tissue Specificity | Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea. |
| Function | Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production. Promotes and augments T-helper type 2 responses locally or systemically. Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction. In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13. Also induces the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways. Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13. |
| Protein Name | Interleukin-25Il-25Interleukin-17eIl-17e |
| Database Links | Reactome: R-HSA-448424 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-25 proteinIl-25 proteinInterleukin-17e proteinIl-17e proteinIL25 proteinIL17E proteinUNQ3120 proteinPRO10272 protein |
Information sourced from Uniprot.org