Human IL-2 (IL-2) Antibody Pair (STJAP00695)
General Information
| Short Description | This IL-2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R08-3Y-4/R08-3G-1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | IL2 |
| Gene ID | 3558 |
| Uniprot ID | IL2_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Function | Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells. |
| Protein Name | Interleukin-2Il-2T-Cell Growth FactorTcgfAldesleukin |
| Database Links | Reactome: R-HSA-5673001Reactome: R-HSA-8877330Reactome: R-HSA-9020558Reactome: R-HSA-912526 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-2 antibody pairIl-2 antibody pairT-Cell Growth Factor antibody pairTcgf antibody pairAldesleukin antibody pairIL2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-2 (IL-2) Antibody Pair (STJAP00695)
This antibody pair detects human Interleukin-2 (IL-2), corresponding to UniProt accession P60568. Reactivity has been confirmed for human samples only.
Both antibodies are rabbit monoclonal IgGs, supplied unconjugated and affinity purified. They are formulated in 0.01M PBS at pH 7.2. Clone IDs are R08-3Y-4 and R08-3G-1 for the two components of the pair.
This antibody pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Working concentrations are to be determined by the end user, as no fixed dilution range is specified. Titration experiments are recommended to establish optimal concentrations for each antibody in the intended assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to preserve antibody integrity.
Both antibodies were raised against a recombinant protein immunogen. The product details do not specify the immunogen region, so researchers intending to detect native human IL-2 in biological samples should validate performance in their specific sample types prior to use.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.