Human IL-2 (IL-2) Antibody Pair (STJAP00660)
General Information
| Short Description | This IL-2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 2A10/12D10 |
| Isotype | IgG1/IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | IL2 |
| Gene ID | 3558 |
| Uniprot ID | IL2_HUMAN |
| Immunogen | A synthesized peptide derived from IL2 |
Additional Info
| Function | Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells. |
| Protein Name | Interleukin-2Il-2T-Cell Growth FactorTcgfAldesleukin |
| Database Links | Reactome: R-HSA-5673001Reactome: R-HSA-8877330Reactome: R-HSA-9020558Reactome: R-HSA-912526 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-2 antibody pairIl-2 antibody pairT-Cell Growth Factor antibody pairTcgf antibody pairAldesleukin antibody pairIL2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-2 (IL-2) Antibody Pair (STJAP00660)
This antibody pair detects human Interleukin-2 (IL-2), corresponding to UniProt entry P60568. Reactivity has been confirmed for human samples only.
Both antibodies are mouse monoclonal IgGs supplied as unconjugated, affinity-purified liquids at 1 mg/mL. The capture antibody (clone 2A10, IgG1) is formulated in PBS with 0.09% sodium azide, and the detection antibody (clone 12D10, IgG2a) is formulated in PBS with 0.01% thimerosal.
This pair has been validated for use in ELISA at a dilution of 1:1000. No other assay formats are specified for this product.
Both antibodies were raised against a synthesized peptide derived from human IL-2. The specific immunogen region is not detailed in the product specifications.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain reagent integrity.
Yes, both antibodies are supplied unconjugated, so the detection antibody will require conjugation to a reporter enzyme or label by the end user prior to use in a sandwich ELISA format.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.