Human IL-17F (IL-17F) Antibody Pair (STJAP00964)
General Information
| Short Description | This IL-17F antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | IL17F |
| Gene ID | 112744 |
| Uniprot ID | IL17F_HUMAN |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. IL17A-IL17F signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter through SEFIR domains. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. IL17A-IL17F is primarily involved in host defense against extracellular bacteria and fungi by inducing neutrophilic inflammation. As signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. Stimulates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers. IL17F homodimer can signal via IL17RC homodimeric receptor complex, triggering downstream activation of TRAF6 and NF-kappa-B signaling pathway. Via IL17RC induces transcriptional activation of IL33, a potent cytokine that stimulates group 2 innate lymphoid cells and adaptive T-helper 2 cells involved in pulmonary allergic response to fungi. Likely via IL17RC, promotes sympathetic innervation of peripheral organs by coordinating the communication between gamma-delta T cells and parenchymal cells. Stimulates sympathetic innervation of thermogenic adipose tissue by driving TGFB1 expression. Regulates the composition of intestinal microbiota and immune tolerance by inducing antimicrobial proteins that specifically control the growth of commensal Firmicutes and Bacteroidetes. |
| Protein Name | Interleukin-17fIl-17fCytokine Ml-1 |
| Database Links | Reactome: R-HSA-448424Reactome: R-HSA-6785807Reactome: R-HSA-9705671 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interleukin-17f antibody pairIl-17f antibody pairCytokine Ml-1 antibody pairIL17F antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IL-17F (IL-17F) Antibody Pair (STJAP00964)
This antibody pair detects human IL-17F (UniProt Q96PD4) and has confirmed reactivity with human samples only.
The pair consists of two goat-derived antibodies: the capture antibody is unconjugated and supplied at 0.5 mg/mL, while the detection antibody is biotin-conjugated and supplied at 0.5 to 4 ug/mL.
This pair has been validated for use in sandwich ELISA using a colorimetric detection method, and is suitable for measuring IL-17F in serum, plasma, and other biological fluids.
A working dilution range of 1:1000 to 1:10000 is recommended, though users should determine the optimal dilution empirically for their specific experimental conditions.
Both components should be stored at -20 degrees Celsius for long-term storage, and repeated freeze-thaw cycles should be avoided to maintain activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.