Human IL-15RA/CD215 protein (Recombinant) (C-hFc&His) (STJP001206)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human IL-15RA/CD215 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Thr205) of human IL-15R alpha/CD215 (Accession #NP_002180.1) fused with a Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human IL-15RA/CD215-C-hFc&His protein was developed in hek293 cells using the region Met1-Thr205. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IL15 at 2 Mu g/mL (100 Mu L/well) can bind recombinant Human IL-15R alpha, the EC50 of Human IL-15R alpha is 178.9 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IL15RA |
| Gene ID | 3601 |
| Uniprot ID | I15RA_HUMAN |
| Immunogen | Recombinant Human IL-15RA/CD215 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Thr205) of human IL-15R alpha/CD215 (Accession #NP_002180.1) fused with a Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Met1-Thr205 |
| Immunogen Sequence | MAPRRARGCRTLGLPALLLL LLLRPPATRGITCPPPMSVE HADIWVKSYSLYSRERYICN SGFKRKAGTSSLTECVLNKA TNVAHWTTPSLKCIRDPALV HQRPAPPSTVTTAGVTPQPE SLSPSGKEPAASSPSSNNTA ATTAAIVPGSQLMPSKSPST GTTEISSHESSHGTPSQTTA KNWELTASASHQPPGVYPQG HSDTT |
Additional Info
| Post Translational Modifications | N-glycosylated and O-glycosylated. A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor. |
| Function | High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. In neutrophils, binds and activates kinase SYK in response to IL15 stimulation. In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5: Does not bind IL15. Isoform 6: Does not bind IL15. Isoform 7: Does not bind IL15. Isoform 8: Does not bind IL15. |
| Protein Name | Interleukin-15 Receptor Subunit AlphaIl-15 Receptor Subunit AlphaIl-15r-AlphaIl-15raCd Antigen Cd215 Cleaved Into - Soluble Interleukin-15 Receptor Subunit AlphaSil-15 Receptor Subunit AlphaSil-15r-AlphaSil-15ra |
| Database Links | Reactome: R-HSA-8983432 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinNucleus MembraneCell SurfaceMainly Found Associated With The Nuclear MembraneIsoform 5: Endoplasmic Reticulum MembraneGolgi Apparatus MembraneCytoplasmic Vesicle MembraneIsoform 5Isoform 6Isoform 7 And Isoform 8 Are Associated With Endoplasmic ReticulumGolgi And Cytoplasmic VesiclesBut Not With The Nuclear MembraneIsoform 6: Endoplasmic Reticulum MembraneIsoform 7: Endoplasmic Reticulum MembraneIsoform 8: Endoplasmic Reticulum MembraneSoluble Interleukin-15 Receptor Subunit Alpha: SecretedExtracellular Space |
| Alternative Protein Names | Interleukin-15 Receptor Subunit Alpha proteinIl-15 Receptor Subunit Alpha proteinIl-15r-Alpha proteinIl-15ra proteinCd Antigen Cd215 Cleaved Into - Soluble Interleukin-15 Receptor Subunit Alpha proteinSil-15 Receptor Subunit Alpha proteinSil-15r-Alpha proteinSil-15ra proteinIL15RA protein |
Information sourced from Uniprot.org