| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human IL-12B p40-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution pH 7.4. |
| Dilution Range: |
Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity was determined by its ability to bind recombinant Human IL-12RB1 in functional ELISA. NA |
| Gene Symbol: |
IL12B |
| Gene ID: |
3593 |
| Uniprot ID: |
IL12B_HUMAN |
| Immunogen Region: |
ECD |
| Immunogen: |
DNA sequence encoding extracellular domain of Human IL-12B fused to a C-terminal polyHis tag wasexpressed in HEK cells. NA |
| Post Translational Modifications | Known to be C-mannosylated in the recombinant protein.it is not yet known for sure if the wild-type protein is also modified. |
| Function | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. |
| Protein Name | Interleukin-12 Subunit BetaIl-12bCytotoxic Lymphocyte Maturation Factor 40 Kda SubunitClmf P40Il-12 Subunit P40Nk Cell Stimulatory Factor Chain 2Nksf2 |
| Database Links | Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9020591Reactome: R-HSA-9020933 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-12 Subunit Beta proteinIl-12b proteinCytotoxic Lymphocyte Maturation Factor 40 Kda Subunit proteinClmf P40 proteinIl-12 Subunit P40 proteinNk Cell Stimulatory Factor Chain 2 proteinNksf2 proteinIL12B proteinNKSF2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
