Human IL-12A protein (Recombinant) (C-hFc&His) (STJP000948)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human IL-12A Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg23-Ser219) of human IL12A/NKSF1 (Accession #NP_002178.2) fused with an Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human IL-12A-C-hFc&His protein was developed in hek293 cells using the region Arg23-Ser219. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IL12A |
| Gene ID | 3592 |
| Uniprot ID | IL12A_HUMAN |
| Immunogen | Recombinant Human IL-12A Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg23-Ser219) of human IL12A/NKSF1 (Accession #NP_002178.2) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Arg23-Ser219 |
| Immunogen Sequence | RNLPVATPDPGMFPCLHHSQ NLLRAVSNMLQKARQTLEFY PCTSEEIDHEDITKDKTSTV EACLPLELTKNESCLNSRET SFITNGSCLASRKTSFMMAL CLSSIYEDLKMYQVEFKTMN AKLLMDPKRQIFLDQNMLAV IDELMQALNFNSETVPQKSS LEEPDFYKTKIKLCILLHAF RIRAVTIDRVMSYLNAS |
Additional Info
| Function | Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity. Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes. As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine. Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites. |
| Protein Name | Interleukin-12 Subunit AlphaIl-12aCytotoxic Lymphocyte Maturation Factor 35 Kda SubunitClmf P35Il-12 Subunit P35Nk Cell Stimulatory Factor Chain 1Nksf1 |
| Database Links | Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-8984722Reactome: R-HSA-9020591 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-12 Subunit Alpha proteinIl-12a proteinCytotoxic Lymphocyte Maturation Factor 35 Kda Subunit proteinClmf P35 proteinIl-12 Subunit P35 proteinNk Cell Stimulatory Factor Chain 1 proteinNksf1 proteinIL12A proteinNKSF1 protein |
Information sourced from Uniprot.org