Human IL-12/IL-12A & IL-12B protein (Recombinant) (C-His (IL12B) & C-Flag (IL12A)) (STJP002737)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenActive Recombinant Human IL-12A&IL-12B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile23-Ser328&Arg23-Ser219) of human IL-12 (Accession #NP_002178.2/NP_000873.2) fused with a 6×His tag at the
General Information
| Short Description | Recombinant-Human IL-12/IL-12A & IL-12B-C-His (IL12B) & C-Flag (IL12A) protein was developed in hek293 cells using the region Ile23-Ser328&Arg23-Ser219. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL-12 at 1 Mu g/mL (100 Mu L/well) can bind Recombinant Human IL12R Beta 1 with a linear range of 30.6-122.4 ng/mL.|2.Measured in a cell proliferation assay using |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IL12BIL12A |
| Gene ID | 35933592 |
| Uniprot ID | IL12B_HUMANIL12A_HUMAN |
| Immunogen | Active Recombinant Human IL-12A&IL-12B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile23-Ser328&Arg23-Ser219) of human IL-12 (Accession #NP_002178.2/NP_000873.2) fused with a 6×His tag at the |
| Immunogen Region | Ile23-Ser328&Arg23-Ser219 |
| Immunogen Sequence | IWELKKDVYVVELDWYPDAP GEMVVLTCDTPEEDGITWTL DQSSEVLGSGKTLTIQVKEF GDAGQYTCHKGGEVLSHSLL LLHKKEDGIWSTDILKDQKE PKNKTFLRCEAKNYSGRFTC WWLTTISTDLTFSVKSSRGS SDPQGVTCGAATLSAERVRG DNKEYEYSVECQEDSACPAA EESLPIEVMVDAVHKLKYEN YTSSFFIRDIIKPDPPKNLQ LKPLKNSRQVEVSWEYPDT |