Human IL-10 (Interleukin-10) Antibody Pair (STJAP00037)

SPECIFICATIONS
ClonalityMonoclonal/Polyclonal
HostMouse/Goat
ConjugationUnconjugated/Biotin
ImmunogenCapture immunogen: Recombinant Human IL-10 protein.
Detection Immunogen: Recombinant Human IL-10 protein.
STJAP00037-50
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General Information

Short DescriptionThis IL-10 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA.
ApplicationsELISA
HostMouse/Goat
ReactivityHuman
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

ClonalityMonoclonal/Polyclonal
ConjugationUnconjugated/Biotin
Concentration0.5 mg/mL
PurificationCapture Purification: Protein A or G.
Detection Purification: Antigen Affinity.
FormulationCapture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.
Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.
Storage InstructionStore at-20°C for the long term, and avoid freeze-thaw cycles.

Target Information

Gene SymbolIL10
Gene ID3586
Uniprot IDIL10_HUMAN
ImmunogenCapture immunogen: Recombinant Human IL-10 protein.
Detection Immunogen: Recombinant Human IL-10 protein.

Additional Info

Function Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
Protein Name Interleukin-10
Il-10
Cytokine Synthesis Inhibitory Factor
Csif
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-8950505
Reactome: R-HSA-9662834
Reactome: R-HSA-9664323
Reactome: R-HSA-9725370
Cellular Localisation Secreted
Alternative Antibody Pair Names Interleukin-10 antibody pair
Il-10 antibody pair
Cytokine Synthesis Inhibitory Factor antibody pair
Csif antibody pair
IL10 antibody pair

Information sourced from Uniprot.org

Citations

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