Human IL-1 Beta (Interleukin-1 Beta) Antibody Pair (STJAP00413)
SPECIFICATIONS
HostMouse/Mouse
ConjugationUnconjugated/Biotin
ImmunogenCapture immunogen: Recombinant Human IL-1 Beta protien.Detection Immunogen: Recombinant Human IL-1 Beta protien.
General Information
| Short Description | This IL-1 Beta antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | IL1B |
| Gene ID | 3553 |
| Uniprot ID | IL1B_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human IL-1 Beta protien.Detection Immunogen: Recombinant Human IL-1 Beta protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. (Microbial infection) Cleavage by S.pyogenes cysteine protease SpeB promotes its activation independently of CASP1. |
| Function | Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection. |
| Protein Name | Interleukin-1 BetaIl-1 BetaCatabolin |
| Database Links | Reactome: R-HSA-448706Reactome: R-HSA-5620971Reactome: R-HSA-5660668Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9020702Reactome: R-HSA-9660826 |
| Cellular Localisation | CytoplasmCytosolSecretedLysosomeExtracellular ExosomeThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsSuch As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 InflammasomeCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Antibody Pair Names | Interleukin-1 Beta antibody pairIl-1 Beta antibody pairCatabolin antibody pairIL1B antibody pairIL1F2 antibody pair |
Information sourced from Uniprot.org