• The purity of Biotinylated Human IL-1 Beta is greater than 95% as determined by SEC-HPLC.
  • Immobilized Human IL-1R2, hFc Tag at 0. 5 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 7. 6ng/ml determined by ELISA.

Human IL-1 Beta/IL-1F2 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001712)

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STJP001712

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human IL-1 Beta/IL-1F2-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: IL1B
Gene ID: 3553
Uniprot ID: IL1B_HUMAN
Immunogen Region: Ala117-Ser269
Immunogen: DNA sequence encoding Human IL-1 Beta/IL-1F2 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. (Microbial infection) Cleavage by S.pyogenes cysteine protease SpeB promotes its activation independently of CASP1.
Function Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection.
Protein Name Interleukin-1 Beta
Il-1 Beta
Catabolin
Database Links Reactome: R-HSA-448706
Reactome: R-HSA-5620971
Reactome: R-HSA-5660668
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-9020702
Reactome: R-HSA-9660826
Cellular Localisation Cytoplasm
Cytosol
Secreted
Lysosome
Extracellular Exosome
The Precursor Is Cytosolic
In Response To Inflammasome-Activating Signals
Such As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 Inflammasome
Cleaved And Secreted
Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore
In Contrast
The Precursor Form Is Not Released
Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
Alternative Protein Names Interleukin-1 Beta protein
Il-1 Beta protein
Catabolin protein
IL1B protein
IL1F2 protein

Information sourced from Uniprot.org

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