Human IgG2 (IgG2) Antibody Pair (STJAP00687)
General Information
| Short Description | This IgG2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 19F4/22C5 |
| Isotype | IgG1/IgG2b |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | IGHG2Uniprot ID=IGHG2_HUMAN" |
| Immunogen | Recombinant human IgG2 |
Additional Info
| Post Translational Modifications | Glycosylation on Asn-176 is required for interaction with Fc receptors and ability to activate the complement pathway. (Microbial infection) Deglycosylation on Asn-176 by S.pyogenes EndoS or Endos2 endoglucosidases prevents interaction between immunoglobulin-gamma (IgG) and Fc receptors, impairing ability to activate the complement pathway. |
| Function | Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. |
| Protein Name | Immunoglobulin Heavy Constant Gamma 2Ig Gamma-2 Chain C RegionIg Gamma-2 Chain C Region DotIg Gamma-2 Chain C Region TilIg Gamma-2 Chain C Region Zie |
| Cellular Localisation | Isoform 1: SecretedIsoform 2: Cell MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Pair Names | Immunoglobulin Heavy Constant Gamma 2 antibody pairIg Gamma-2 Chain C Region antibody pairIg Gamma-2 Chain C Region Dot antibody pairIg Gamma-2 Chain C Region Til antibody pairIg Gamma-2 Chain C Region Zie antibody pairIGHG2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IgG2 (IgG2) Antibody Pair (STJAP00687)
This antibody pair is directed against human IgG2, corresponding to UniProt accession P01859, and has confirmed reactivity with human samples only.
This antibody pair has been validated for use in ELISA. No other assay formats are listed for this product.
Both antibodies are supplied as affinity-purified mouse monoclonal antibodies at 1 mg/mL. The capture antibody (clone 19F4, IgG1) is formulated in PBS with 0.09% sodium azide, and the detection antibody (clone 22C5, IgG2b) is formulated in PBS with 0.01% thimerosal. Neither antibody carries a conjugation or tag.
A 1:1000 dilution is recommended for ELISA applications. Researchers may wish to optimize concentrations further depending on their specific assay conditions.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C, and repeated freeze/thaw cycles should be avoided.
Both antibodies were raised against recombinant human IgG2 in mouse hosts, with clones 19F4 and 22C5 selected for the capture and detection roles respectively.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.