This IGFBP3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications
ELISA
Reactivity
Human
Sensitivity
0.47ng/mL
Detection Limit
0.78~50ng/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Human IGFBP-3 in samples. No significant cross-reactivity or interference between Human IGFBP-3 and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Post Translational Modifications
Phosphorylated by FAM20C in the extracellular medium. Phosphorylated by CK2.resulting in decreased nuclear localization.
Function
Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis. Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1. Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade. In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis.
Protein Name
Insulin-Like Growth Factor-Binding Protein 3Ibp-3Igf-Binding Protein 3Igfbp-3
