Human IGFBP-3 (Insulin-Like Growth Factor-Binding Protein 3) Antibody Pair (STJAP00212)
General Information
| Short Description | This IGFBP-3 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | IGFBP3 |
| Gene ID | 3486 |
| Uniprot ID | IBP3_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human IGFBP-3 protein.Detection Immunogen: Recombinant Human IGFBP-3 protein. |
Additional Info
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. Phosphorylated by CK2.resulting in decreased nuclear localization. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis. Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1. Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade. In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 3Ibp-3Igf-Binding Protein 3Igfbp-3 |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-6803211Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedNucleus |
| Alternative Antibody Pair Names | Insulin-Like Growth Factor-Binding Protein 3 antibody pairIbp-3 antibody pairIgf-Binding Protein 3 antibody pairIgfbp-3 antibody pairIGFBP3 antibody pairIBP3 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human IGFBP-3 (Insulin-Like Growth Factor-Binding Protein 3) Antibody Pair (STJAP00212)
This antibody pair detects human IGFBP-3 (Insulin-Like Growth Factor-Binding Protein 3), corresponding to UniProt accession P17936. Reactivity is confirmed for human samples only.
The capture antibody is a mouse monoclonal antibody supplied unconjugated, while the detection antibody is a goat polyclonal antibody supplied biotinylated. Both components are provided at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human IGFBP-3 protein. The capture antibody was purified by Protein A or G, and the detection antibody was purified by antigen affinity.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.