Human IGFBP-1 protein (Recombinant) (C-His) (STJP001006)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human IGFBP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala26-Asn259) of human IGFBP-1 (Accession #NP_000587.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human IGFBP-1-C-His protein was developed in hek293 cells using the region Ala26-Asn259. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-1 at 5 Mu g/mL (100 Mu L/well) can bind Recombinant Human IGF1 with a linear range of 44-176 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IGFBP1 |
| Gene ID | 3484 |
| Uniprot ID | IBP1_HUMAN |
| Immunogen | Recombinant Human IGFBP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala26-Asn259) of human IGFBP-1 (Accession #NP_000587.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Ala26-Asn259 |
| Immunogen Sequence | APWQCAPCSAEKLALCPPVS ASCSEVTRSAGCGCCPMCAL PLGAACGVATARCARGLSCR ALPGEQQPLHALTRGQGACV QESDASAPHAAEAGSPESPE STEITEEELLDNFHLMAPSE EDHSILWDAISTYDGSKALH VTNIKKWKEPCRIELYRVVE SLAKAQETSGEEISKFYLPN CNKNGFYHSRQCETSMDGEA GLCWCVYPWNGKRIPGSPEI RGDPNCQIYFNVQN |
Additional Info
| Post Translational Modifications | Phosphorylated.probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including cell migration, proliferation, differentiation or apoptosis in a cell-type specific manner. Also plays a positive role in cell migration by interacting with integrin ITGA5:ITGB1 through its RGD motif. Mechanistically, binding to integrins leads to activation of focal adhesion kinase/PTK2 and stimulation of the mitogen-activated protein kinase (MAPK) pathway. Regulates cardiomyocyte apoptosis by suppressing HIF-1alpha/HIF1A ubiquitination and subsequent degradation. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 1Ibp-1Igf-Binding Protein 1Igfbp-1Placental Protein 12Pp12 |
| Database Links | Reactome: R-HSA-380994Reactome: R-HSA-381426Reactome: R-HSA-8957275Reactome: R-HSA-9615017 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Insulin-Like Growth Factor-Binding Protein 1 proteinIbp-1 proteinIgf-Binding Protein 1 proteinIgfbp-1 proteinPlacental Protein 12 proteinPp12 proteinIGFBP1 proteinIBP1 protein |
Information sourced from Uniprot.org