Human IGF-1R (CD221) protein (Recombinant) (STJP000574)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding extracellular domain of human IGF-1R (CD221) including a C-terminal 6His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human IGF-1R (CD221)-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | A quick spin of the vial followed by gently mixing in distilled water to prepare a stock solution concentration not less than 0.1 mg/mL. Avoid vigorous shaking or vortex |
| Formulation | 0.2 Mu m filtered PBS solution, pH7.2. |
| Storage Instruction | Can be stored in working aliquots at 2ยฐC-8ยฐC C for one month, or at-20ยฐC to-70ยฐC for 1 year, addition of a carrier protein to your working aliquots (0.1% BSA or HSA) is recommended for long term storage Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ยตg (1EU/ยตg). NA |
| Immunoreactivity | The activity of recombinant IGF-1R was measured by its ability to bind Human IGF-1 in a functional ELISA assay. NA |
Target Information
| Immunogen | Optimized DNA sequence encoding extracellular domain of human IGF-1R (CD221) including a C-terminal 6His tag was expressed in HEK293 cells. NA |
| Immunogen Region | ECD |
Additional Info
| Background | Recombinant human IGF-1R (CD221) is a heterodimer protein, due to glycosylation migrates as an approximately 50kDa, 120kDa, 150kDa protein under reducing ocnditions on SDS-PAGE. |
Information sourced from Uniprot.org