Human IFN-gamma R1/CD119 protein (Recombinant) (C-His) (STJP002598)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human IFN-gamma R1/CD119 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gly245) of human IFNGR1/CD119 (Accession #NP_000407.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human IFN-gamma R1/CD119-C-His protein was developed in hek293 cells using the region Met1-Gly245. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human FNGR1/CD119 at 1 Mu g/mL (100 Mu L/well) can bind Human IFNG with a linear range of 0.98-1.97 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IFNGR1 |
| Gene ID | 3459 |
| Uniprot ID | INGR1_HUMAN |
| Immunogen | Recombinant Human IFN-gamma R1/CD119 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gly245) of human IFNGR1/CD119 (Accession #NP_000407.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Met1-Gly245 |
| Immunogen Sequence | MALLFLLPLVMQGVSRAEMG TADLGPSSVPTPTNVTIESY NMNPIVYWEYQIMPQVPVFT VEVKNYGVKNSEWIDACINI SHHYCNISDHVGDPSNSLWV RVKARVGQKESAYAKSEEFA VCRDGKIGPPKLDIRKEEKQ IMIDIFHPSVFVNGDEQEVD YDPETTCYIRVYNVYVRMNG SEIQYKILTQKEDDCDEIQC QLAIPVSSLNSQYCVSAEGV LHVWGVTTEKSKEVCITIF |
Additional Info
| Function | Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Associates with transmembrane accessory factor IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription. STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1. |
| Protein Name | Interferon Gamma Receptor 1Ifn-Gamma Receptor 1Ifn-Gamma-R1Cdw119Interferon Gamma Receptor Alpha-ChainIfn-Gamma-R-AlphaCd Antigen Cd119 |
| Database Links | Reactome: R-HSA-877300Reactome: R-HSA-877312Reactome: R-HSA-9679191Reactome: R-HSA-9732724 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Interferon Gamma Receptor 1 proteinIfn-Gamma Receptor 1 proteinIfn-Gamma-R1 proteinCdw119 proteinInterferon Gamma Receptor Alpha-Chain proteinIfn-Gamma-R-Alpha proteinCd Antigen Cd119 proteinIFNGR1 protein |
Information sourced from Uniprot.org