Human ICAM1 (Intercellular adhesion molecule 1) Sandwich ELISA Kit (STJE0004126)

Name: Human ICAM1 (Intercellular adhesion molecule 1) Sandwich ELISA Kit (STJE0004126)
SKU: STJE0004126
Availability: InStock

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SPECIFICATIONS

STJE0004126

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Short Description	This ICAM1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.19ng/mL
Detection Limit	0.31~20ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	ICAM1
Gene ID	3383
Uniprot ID	ICAM1_HUMAN
Specificity	This kit recognizes Human ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Human ICAM-1/CD54 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Protein Name	Intercellular Adhesion Molecule 1 Icam-1 Major Group Rhinovirus Receptor Cd Antigen Cd54
Database Links	Reactome: R-HSA-198933 Reactome: R-HSA-216083 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-877300
Cellular Localisation	Membrane Single-Pass Type I Membrane Protein
Alternative ELISA Names	Intercellular Adhesion Molecule 1 ELISA kit Icam-1 ELISA kit Major Group Rhinovirus Receptor ELISA kit Cd Antigen Cd54 ELISA kit ICAM1 ELISA kit
Specificity	This kit recognizes Human ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Human ICAM-1/CD54 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	1.00	2.42	8.44	0.84	2.29	8.45
Standard deviation	0.06	0.12	0.34	0.05	0.10	0.34
CV (%)	6.00	4.96	4.03	5.95	4.37	4.02