Human ICAM-3/CD50 (Intercellular Adhesion Molecule 3) Antibody Pair (STJAP00399)
General Information
| Short Description | This ICAM-3/CD50 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | ICAM3 |
| Gene ID | 3385 |
| Uniprot ID | ICAM3_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human ICAM-3/CD50 protien.Detection Immunogen: Recombinant Human ICAM-3/CD50 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated.glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages. |
| Protein Name | Intercellular Adhesion Molecule 3Icam-3Cdw50Icam-RCd Antigen Cd50 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-216083Reactome: R-HSA-5621575 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Pair Names | Intercellular Adhesion Molecule 3 antibody pairIcam-3 antibody pairCdw50 antibody pairIcam-R antibody pairCd Antigen Cd50 antibody pairICAM3 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human ICAM-3/CD50 (Intercellular Adhesion Molecule 3) Antibody Pair (STJAP00399)
This antibody pair detects human ICAM-3 (CD50, Intercellular Adhesion Molecule 3), corresponding to UniProt entry P32942. Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated, both at 0.5 mg/mL. Both antibodies are rabbit-derived and purified by antigen affinity chromatography.
This pair is validated for use in ELISA. Recommended sample types include serum, plasma, and other biological fluids.
Both antibodies were raised against recombinant human ICAM-3/CD50 protein. Each antibody was also purified by antigen affinity chromatography using this same recombinant protein.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.