This HVCN1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids..
Applications
ELISA
Reactivity
Human
Sensitivity
0.065ng/mL
Detection Limit
0.156-10ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Enriched in immune tissues, such as lymph nodes, B-lymphocytes, monocytes and spleen. Expressed in spermatozoa. Expressed in respiratory epithelial cells.
Post Translational Modifications
Proteolytically cleaved in sperm. The cleaved protomer forms homodimers and heterodimers with the full-length protomer. It confers reduced dependence to intracellular pH changes. Phosphorylated in vitro by PRKCD. Phosphorylation may enhance channel gating.
Function
Voltage-gated proton-selective channel that conducts outward proton currents in response to intracellular acidification. Lacks a canonical ion-channel pore domain and mediates proton permeability via its voltage sensor domain. Appears to play a dominant role in regulation of CO2/HCO3(-)/H(+) equilibrium in sperm flagellum. Prevents the acidification resulting from HCO3(-) synthesis and thus sustains high HCO3(-) levels inside sperm for capacitation. Provides for proton efflux that compensates for electron charge generated by NADPH oxidase activity either in the extracellular or phagosomal compartments, thus enabling the production of high levels of bactericidal reactive oxygen species during the respiratory burst. Opens when the pH of airway surface liquid exceeds 7 and contributes to respiratory epithelial acid secretion to maintain pH in the mucosa.
Protein Name
Voltage-Gated Hydrogen Channel 1Hydrogen Voltage-Gated Channel 1Hv1Voltage Sensor Domain-Only Protein
Cell MembraneMulti-Pass Membrane ProteinApical Cell MembraneCytoplasmic VesiclePhagosome MembraneCell ProjectionCiliumFlagellum MembraneDetected Within The Principal Piece Of The Sperm FlagellumDetected Mainly At Intracellular Membranes Upon Overexpression In Hela Cells
