Human HTRA2 protein (Recombinant) (No tag) (STJP000816)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Human HTRA2 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala134-Glu458) of human HTRA2/Omi (Accession #NP_037379.1).
General Information
| Short Description | Recombinant-Human HTRA2-No tag protein was developed in e.coli using the region Ala134-Glu458. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Protease activity demonstrated by HtrA2 cleavage of bovine Beta-casein (Sigma, Catalog # C-6905). Incubation of Beta-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45 °C in 50 mM Tris, pH 8.0, which |
| Determination Method | Please contact us for more information. |
Target Information
| Gene Symbol | HTRA2 |
| Gene ID | 27429 |
| Uniprot ID | HTRA2_HUMAN |
| Immunogen | Recombinant Human HTRA2 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala134-Glu458) of human HTRA2/Omi (Accession #NP_037379.1). |
| Immunogen Region | Ala134-Glu458 |
| Immunogen Sequence | AVPSPPPASPRSQYNFIADV VEKTAPAVVYIEILDRHPFL GREVPISNGSGFVVAADGLI VTNAHVVADRRRVRVRLLSG DTYEAVVTAVDPVADIATLR IQTKEPLPTLPLGRSADVRQ GEFVVAMGSPFALQNTITSG IVSSAQRPARDLGLPQTNVE YIQTDAAIDFGNSGGPLVNL DGEVIGVNTMKVTAGISFAI PSDRLREFLHRGEKKNSSSG ISGSQRRYIGVMMLTLSPS |
Additional Info
| Tissue Specificity | Isoform 1: Ubiquitously expressed. |
| Post Translational Modifications | Ubiquitinated by BIRC6.this activity is inhibited by DIABLO/SMAC. Autoproteolytically activated. |
| Function | Isoform 1: Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity.or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2: Seems to be proteolytically inactive. |
| Protein Name | Serine Protease Htra2 - MitochondrialHigh Temperature Requirement Protein A2Htra2Omi Stress-Regulated EndoproteaseSerine Protease 25Serine Proteinase Omi |
| Database Links | Reactome: R-HSA-9837999Reactome: R-HSA-9841251 |
| Cellular Localisation | Mitochondrion Intermembrane SpaceMitochondrion MembraneSingle-Pass Membrane ProteinPredominantly Present In The Intermembrane SpaceReleased Into The Cytosol Following Apoptotic StimuliSuch As Uv TreatmentAnd Stimulation Of Mitochondria With Caspase-8 Truncated Bid/TbidIsoform 1: Endoplasmic Reticulum |
| Alternative Protein Names | Serine Protease Htra2 - Mitochondrial proteinHigh Temperature Requirement Protein A2 proteinHtra2 proteinOmi Stress-Regulated Endoprotease proteinSerine Protease 25 proteinSerine Proteinase Omi proteinHTRA2 proteinOMI proteinPRSS25 protein |
Information sourced from Uniprot.org