Human HSPA9 (Stress-70 protein, mitochondrial) Sandwich ELISA Kit (STJE0003280)

Name: Human HSPA9 (Stress-70 protein, mitochondrial) Sandwich ELISA Kit (STJE0003280)
SKU: STJE0003280
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0003280

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Short Description	This HSPA9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.47ng/mL
Detection Limit	0.78~50ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	HSPA9
Gene ID	3313
Uniprot ID	HSPA9_HUMAN
Specificity	This kit recognizes Human HSP-70/HSPA9 in samples. No significant cross-reactivity or interference between Human HSP-70/HSPA9 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Protein Name	Stress-70 Protein - Mitochondrial 75 Kda Glucose-Regulated Protein Grp-75 Heat Shock 70 Kda Protein 9 Heat Shock Protein Family A Member 9 Mortalin Mot Peptide-Binding Protein 74 Pbp74
Database Links	Reactome: R-HSA-1268020 Reactome: R-HSA-3371453 Reactome: R-HSA-6799198 Reactome: R-HSA-8949613 Reactome: R-HSA-8950505 Reactome: R-HSA-9837999 Reactome: R-HSA-9841251 Reactome: R-HSA-9865881
Cellular Localisation	Mitochondrion Nucleus Nucleolus Cytoplasm Mitochondrion Matrix Found In A Complex With Hspa9 And Vdac1 At The Endoplasmic Reticulum-Mitochondria Contact Sites
Alternative ELISA Names	Stress-70 Protein - Mitochondrial ELISA kit 75 Kda Glucose-Regulated Protein ELISA kit Grp-75 ELISA kit Heat Shock 70 Kda Protein 9 ELISA kit Heat Shock Protein Family A Member 9 ELISA kit Mortalin ELISA kit Mot ELISA kit Peptide-Binding Protein 74 ELISA kit Pbp74 ELISA kit HSPA9 ELISA kit GRP75 ELISA kit HSPA9B ELISA kit mt-HSP70 ELISA kit
Specificity	This kit recognizes Human HSP-70/HSPA9 in samples. No significant cross-reactivity or interference between Human HSP-70/HSPA9 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	2.30	6.00	22.60	2.30	5.60	24.10
Standard deviation	0.10	0.30	1.10	0.10	0.30	0.90
CV (%)	4.35	5.00	4.87	4.35	5.36	3.73