SDS-PAGE of recombinant human HSP27 protein under reducing conditions with Coomassie blue staining

Human HSP27 protein (Recombinant) (No-Tag) (STJP019658)

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STJP019658
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Short Description :Recombinant-Human HSP27-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications.
Applications:SDS-PAGE
Host:E.coli
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Concentration:1 mg/mL
Formulation:Liquid in 20mM HEPES buffer (pH 7.4) containing 100mM KCl, 1mM DTT
Storage Instruction:For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Endotoxin:< 1 EU per 1ug of protein (determined by LAL method)
Gene Symbol:HSPB1
Gene ID:3315
Uniprot ID:HSPB1_HUMAN
Accession Number:NP_001531
Immunogen:Human
Immunogen Region:1-205aa
Immunogen Sequence:MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK
Tissue Specificity Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Post Translational Modifications Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.
Function Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
Protein Name Heat Shock Protein Beta-1
Hspb1
28 Kda Heat Shock Protein
Estrogen-Regulated 24 Kda Protein
Heat Shock 27 Kda Protein
Hsp 27
Heat Shock Protein Family B Member 1
Stress-Responsive Protein 27
Srp27
Database Links Reactome: R-HSA-4420097
Reactome: R-HSA-450408
Reactome: R-HSA-5687128
Reactome: R-HSA-9009391
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Spindle
Cytoplasmic In Interphase Cells
Colocalizes With Mitotic Spindles In Mitotic Cells
Translocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles
Alternative Protein Names Heat Shock Protein Beta-1 protein
Hspb1 protein
28 Kda Heat Shock Protein protein
Estrogen-Regulated 24 Kda Protein protein
Heat Shock 27 Kda Protein protein
Hsp 27 protein
Heat Shock Protein Family B Member 1 protein
Stress-Responsive Protein 27 protein
Srp27 protein
HSPB1 protein
HSP27 protein
HSP28 protein

Information sourced from Uniprot.org

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