Human HPRT protein (Recombinant) (His-Tag) (STJP019298)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human HPRT-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | Enzyme Activity/SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing, 20% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity is > 15unit/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C. |
Target Information
| Gene Symbol | HPRT1 |
| Gene ID | 3251 |
| Uniprot ID | HPRT_HUMAN |
| Accession Number | NP_000185 |
| Immunogen | Human |
| Immunogen Region | 1-218aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVI |
Additional Info
| Function | Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. |
| Protein Name | Hypoxanthine-Guanine PhosphoribosyltransferaseHgprtHgprtase |
| Database Links | Reactome: R-HSA-74217Reactome: R-HSA-9734281Reactome: R-HSA-9748787 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Hypoxanthine-Guanine Phosphoribosyltransferase proteinHgprt proteinHgprtase proteinHPRT1 proteinHPRT protein |
Information sourced from Uniprot.org