Human HO1 (Heme Oxygenase 1) Antibody Pair (STJAP00456)
General Information
| Short Description | This HO1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | HMOX1 |
| Gene ID | 3162 |
| Uniprot ID | HMOX1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human HO1 protien.Detection Immunogen: Recombinant Human HO1 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | A soluble form arises by proteolytic removal of the membrane anchor. |
| Function | Heme oxygenase 1: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis. Heme oxygenase 1: (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. Heme oxygenase 1 soluble form: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. |
| Protein Name | Heme Oxygenase 1Ho-1 Cleaved Into - Heme Oxygenase 1 Soluble Form |
| Database Links | Reactome: R-HSA-189483Reactome: R-HSA-6785807Reactome: R-HSA-844456Reactome: R-HSA-917937Reactome: R-HSA-9609523Reactome: R-HSA-9660826Reactome: R-HSA-9707564Reactome: R-HSA-9707587Reactome: R-HSA-9707616Reactome: R-HSA-9818027 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Iv Membrane ProteinCytoplasmic Side |
| Alternative Antibody Pair Names | Heme Oxygenase 1 antibody pairHo-1 Cleaved Into - Heme Oxygenase 1 Soluble Form antibody pairHMOX1 antibody pairHO antibody pairHO1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human HO1 (Heme Oxygenase 1) Antibody Pair (STJAP00456)
This antibody pair detects Human Heme Oxygenase 1 (HO1, UniProt P09601) and is validated for reactivity with human samples only. No cross-reactivity data for other species is provided.
This antibody pair is validated for use in ELISA. Compatible sample types include serum, plasma, and other biological fluids.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated, both at 0.5 mg/mL. Both are rabbit-raised and purified by antigen affinity. The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both the capture and detection antibodies were raised against recombinant Human HO1 protein. No specific immunogen region or fragment details are provided in the product information.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.