Human HMGCL protein (Recombinant) (His-Tag) (STJP017468)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human HMGCL-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 0.4M urea |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HMGCL |
| Gene ID | 3155 |
| Uniprot ID | HMGCL_HUMAN |
| Accession Number | NP_000182 |
| Immunogen | Human |
| Immunogen Region | 28-325aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMTLPKR VKIVEVGPRD GLQNEKNIVS TPVKIKLIDM LSEAGLSVIE TTSFVSPKWV PQMGDHTEVL KGIQKFPGIN YPVLTPNLKG FEAAVAAGAK EVVIFGAASE LFTKKNINCS IEESFQRFDA ILKAAQSANI SVRGYVSCAL GCPYEGKISP AKVAEVTKKF YSMGCYEISL GDTIGVGTPG IMKDMLSAVM QEVPLAAL |
Additional Info
| Tissue Specificity | Highest expression in liver. Expressed in pancreas, kidney, intestine, testis, fibroblasts and lymphoblasts. Very low expression in brain and skeletal muscle. The relative expression of isoform 2 (at mRNA level) is highest in heart (30%), skeletal muscle (22%), and brain (14%). |
| Function | Mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism. |
| Protein Name | Hydroxymethylglutaryl-Coa Lyase - MitochondrialHlHmg-Coa Lyase3-Hydroxy-3-Methylglutarate-Coa Lyase |
| Database Links | Reactome: R-HSA-77111Reactome: R-HSA-9033241 |
| Cellular Localisation | Mitochondrion MatrixPeroxisomeUnprocessed Form Is Peroxisomal |
| Alternative Protein Names | Hydroxymethylglutaryl-Coa Lyase - Mitochondrial proteinHl proteinHmg-Coa Lyase protein3-Hydroxy-3-Methylglutarate-Coa Lyase proteinHMGCL protein |
Information sourced from Uniprot.org