Human HMGB3 protein (Recombinant) (C-His) (STJP012974)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human HMGB3-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a 0.22 Mu m filtered solution in 20mM PB, 8% Trehalose, 2% Mannitol, 0.05% Tween 80, 2mM EDTA, pH 7.0. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | HMGB3 |
| Gene ID | 3149 |
| Uniprot ID | HMGB3_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Glu200 |
Additional Info
| Post Translational Modifications | Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so). |
| Function | Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling. |
| Protein Name | High Mobility Group Protein B3High Mobility Group Protein 2aHmg-2aHigh Mobility Group Protein 4Hmg-4 |
| Database Links | |
| Cellular Localisation | NucleusChromosomeCytoplasm |
| Alternative Protein Names | High Mobility Group Protein B3 proteinHigh Mobility Group Protein 2a proteinHmg-2a proteinHigh Mobility Group Protein 4 proteinHmg-4 proteinHMGB3 proteinHMG2A proteinHMG4 protein |
Information sourced from Uniprot.org