Human HMBS protein (Recombinant) (His-Tag) (STJP017556)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human HMBS-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol, 0.1M NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HMBS |
| Gene ID | 3145 |
| Uniprot ID | HEM3_HUMAN |
| Accession Number | NP_000181 |
| Immunogen | Human |
| Immunogen Region | 1-361aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMSGNGN AAATAEENSP KMRVIRVGTR KSQLARIQTD SVVATLKASY PGLQFEIIAM STTGDKILDT ALSKIGEKSL FTKELEHALE KNEVDLVVHS LKDLPTVLPP GFTIGAICKR ENPHDAVVFH PKFVGKTLET LPEKSVVGTS SLRRAAQLQR KFPHLEFRSI RGNLNTRLRK LDEQQEFSAI ILATAGLQRM GWHNRVGQ |
Additional Info
| Tissue Specificity | Isoform 1: Is ubiquitously expressed. Isoform 2: Is found only in erythroid cells. |
| Function | As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen. Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact. |
| Protein Name | Porphobilinogen DeaminasePbg-DHydroxymethylbilane SynthaseHmbsPre-Uroporphyrinogen Synthase |
| Database Links | Reactome: R-HSA-189451 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Protein Names | Porphobilinogen Deaminase proteinPbg-D proteinHydroxymethylbilane Synthase proteinHmbs proteinPre-Uroporphyrinogen Synthase proteinHMBS proteinPBGD proteinUPS protein |
Information sourced from Uniprot.org