Human HLA-DR (HLA-DR) Antibody Pair (STJAP00874)
General Information
| Short Description | This HLA-DR antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R09-1F-6/R09-5F-2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | HLA-DRA |
| Gene ID | 3122 |
| Uniprot ID | DRA_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in professional APCs: macrophages, dendritic cells and B cells (at protein level). Expressed in thymic epithelial cells (at protein level). |
| Post Translational Modifications | Ubiquitinated by MARCHF1 or MARCHF8 at Lys-244 leading to down-regulation of MHCII. When associated with ubiquitination of the beta chain at 'Lys-254', the down-regulation of MHCII may be highly effective. |
| Function | An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance. The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides. The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules. |
| Protein Name | Hla Class Ii Histocompatibility Antigen - Dr Alpha ChainMhc Class Ii Antigen Dra |
| Database Links | Reactome: R-HSA-202424Reactome: R-HSA-202427Reactome: R-HSA-202430Reactome: R-HSA-202433Reactome: R-HSA-2132295Reactome: R-HSA-389948Reactome: R-HSA-877300 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum MembraneEarly Endosome MembraneLate Endosome MembraneLysosome MembraneAutolysosome MembraneThe Mhcii Complex Transits Through A Number Of Intracellular Compartments In The Endocytic Pathway Until It Reaches The Cell Membrane For Antigen PresentationComponent Of Immunological Synapses At The Interface Between T Cell And Apc |
| Alternative Antibody Pair Names | Hla Class Ii Histocompatibility Antigen - Dr Alpha Chain antibody pairMhc Class Ii Antigen Dra antibody pairHLA-DRA antibody pairHLA-DRA1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human HLA-DR (HLA-DR) Antibody Pair (STJAP00874)
This antibody pair is validated to detect human HLA-DR, corresponding to UniProt entry P01903. Reactivity has been confirmed for human samples only.
This antibody pair is validated for use in ELISA. Both antibodies were raised against recombinant protein immunogen, making them suitable for sandwich ELISA development.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated and formulated in 0.01M PBS at pH 7.2. They have been affinity purified, and working concentrations are to be determined by the end user.
The two antibodies in this pair are clones R09-1F-6 and R09-5F-2, both rabbit monoclonal IgGs. Both were generated using a recombinant protein immunogen.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.