Human HK1 (Hexokinase 1) Sandwich ELISA Kit Ready-To-Use Kit (STJE0015848)
SPECIFICATIONS
General Information
| Short Description | This HK1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 27.2pg/mL |
| Detection Limit | 62.5-4000pg/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | HK1 |
| Gene ID | 3098 |
| Uniprot ID | HXK1_HUMAN |
| Immunogen Region | Ready-To-Use |
| Sample Type | serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific. Isoform 4: Testis-specific. |
| Post Translational Modifications | |
| Function | Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome. |
| Protein Name | Hexokinase-1Brain Form HexokinaseHexokinase Type IHk IHexokinase-A |
| Database Links | Reactome: R-HSA-446205Reactome: R-HSA-5619056Reactome: R-HSA-70171 |
| Cellular Localisation | Mitochondrion Outer MembranePeripheral Membrane ProteinCytoplasmCytosolThe Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer Membrane (Probable)Dissociates From The Mitochondrial Outer Membrane Following Inhibition By N-Acetyl-D-GlucosamineLeading To Relocation To The Cytosol |
| Alternative ELISA Names | Hexokinase-1 ELISA kitBrain Form Hexokinase ELISA kitHexokinase Type I ELISA kitHk I ELISA kitHexokinase-A ELISA kitHK1 ELISA kit |
| output |
Information sourced from Uniprot.org