Human HINT1 protein (Recombinant) (N-His) (STJP007428)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human HINT1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | HINT1 |
| Gene ID | 3094 |
| Uniprot ID | HINT1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Gly126 |
Additional Info
| Function | Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17. Deconjugates SUMO1 from RANGAP1. |
| Protein Name | Adenosine 5'-Monophosphoramidase Hint1Desumoylating Isopeptidase Hint1Histidine Triad Nucleotide-Binding Protein 1Protein Kinase C Inhibitor 1Protein Kinase C-Interacting Protein 1Pkci-1 |
| Database Links | Reactome: R-HSA-9824594Reactome: R-HSA-9825892Reactome: R-HSA-9856649 |
| Cellular Localisation | CytoplasmNucleusInteraction With Cdk7 Leads To A More Nuclear Localization |
| Alternative Protein Names | Adenosine 5'-Monophosphoramidase Hint1 proteinDesumoylating Isopeptidase Hint1 proteinHistidine Triad Nucleotide-Binding Protein 1 proteinProtein Kinase C Inhibitor 1 proteinProtein Kinase C-Interacting Protein 1 proteinPkci-1 proteinHINT1 proteinHINT proteinPKCI1 proteinPRKCNH1 protein |
Information sourced from Uniprot.org