Human HIF-1 Alpha (Hypoxia-Inducible Factor 1-Alpha) Antibody Pair (STJAP00419)
SPECIFICATIONS
HostRabbit/Goat
ConjugationUnconjugated/Biotin
ImmunogenCapture immunogen: Recombinant Human HIF-1 Alpha protien.Detection Immunogen: Recombinant Human HIF-1 Alpha protien.
General Information
| Short Description | This HIF-1 Alpha antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | HIF1A |
| Gene ID | 3091 |
| Uniprot ID | HIF1A_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human HIF-1 Alpha protien.Detection Immunogen: Recombinant Human HIF-1 Alpha protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex. Requires phosphorylation for DNA-binding. Phosphorylation at Ser-247 by CSNK1D/CK1 represses kinase activity and impairs ARNT binding. Phosphorylation by GSK3-beta and PLK3 promote degradation by the proteasome. Sumoylated.with SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Both sumoylation and desumoylation seem to be involved in the regulation of its stability during hypoxia. Sumoylation can promote either its stabilization or its VHL-dependent degradation by promoting hydroxyproline-independent HIF1A-VHL complex binding, thus leading to HIF1A ubiquitination and proteasomal degradation. Desumoylation by SENP1 increases its stability amd transcriptional activity. There is a disaccord between various publications on the effect of sumoylation and desumoylation on its stability and transcriptional activity (Probable). Acetylation of Lys-532 by ARD1 increases interaction with VHL and stimulates subsequent proteasomal degradation. Deacetylation of Lys-709 by SIRT2 increases its interaction with and hydroxylation by EGLN1 thereby inactivating HIF1A activity by inducing its proteasomal degradation. Polyubiquitinated.in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803. Ubiquitinated by E3 ligase VHL. Deubiquitinated by UCHL1. In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD2 and EGLN2/PHD1. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol. Repressed by iron ion, via Fe(2+) prolyl hydroxylase (PHD) enzymes-mediated hydroxylation and subsequent proteasomal degradation. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. (Microbial infection) Glycosylated at Arg-18 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation enhances transcription factor activity and impairs glucose metabolism. |
| Function | Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT.heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, is required for induction of glycolysis in monocytes and the consequent pro-inflammatory state. In monocytes, induces expression of ACE2 and cytokines such as IL1B, TNF, IL6, and interferons. Promotes human coronavirus SARS-CoV-2 replication and monocyte inflammatory response. |
| Protein Name | Hypoxia-Inducible Factor 1-AlphaHif-1-AlphaHif1-AlphaArnt-Interacting ProteinBasic-Helix-Loop-Helix-Pas Protein Mop1Class E Basic Helix-Loop-Helix Protein 78Bhlhe78Member Of Pas Protein 1Pas Domain-Containing Protein 8 |
| Database Links | Reactome: R-HSA-1234158Reactome: R-HSA-1234174Reactome: R-HSA-1234176Reactome: R-HSA-2122947Reactome: R-HSA-400253Reactome: R-HSA-5689880Reactome: R-HSA-6785807Reactome: R-HSA-8849473Reactome: R-HSA-8857538Reactome: R-HSA-8951664Reactome: R-HSA-9701898 |
| Cellular Localisation | CytoplasmNucleusNucleus SpeckleColocalizes With Hif3a In The Nucleus And SpecklesCytoplasmic In NormoxiaNuclear Translocation In Response To Hypoxia |
| Alternative Antibody Pair Names | Hypoxia-Inducible Factor 1-Alpha antibody pairHif-1-Alpha antibody pairHif1-Alpha antibody pairArnt-Interacting Protein antibody pairBasic-Helix-Loop-Helix-Pas Protein Mop1 antibody pairClass E Basic Helix-Loop-Helix Protein 78 antibody pairBhlhe78 antibody pairMember Of Pas Protein 1 antibody pairPas Domain-Containing Protein 8 antibody pairHIF1A antibody pairBHLHE78 antibody pairMOP1 antibody pairPASD8 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human HIF-1 Alpha (Hypoxia-Inducible Factor 1-Alpha) Antibody Pair (STJAP00419)
This antibody pair detects human HIF-1 Alpha (Hypoxia-Inducible Factor 1-Alpha), corresponding to UniProt entry Q16665. Reactivity is confirmed for human samples only.
The capture antibody is unconjugated and rabbit-derived, while the detection antibody is biotin-conjugated and goat-derived. Both components are supplied at 0.5 mg/mL and are purified by antigen affinity chromatography.
This pair is validated for use in ELISA. Recommended sample types include serum, plasma, and other biological fluids.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Both the capture and detection antibodies were raised against recombinant human HIF-1 Alpha protein. Both antibodies have been purified by antigen affinity chromatography.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.