• Biotinylated Human HGF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human HGF R is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-HGF R Antibody, hFc Tag at 0. 5 Mu g/ml (100 Mu l/Well) on the plate. Dose response curve for Biotinylated Human HGF R, His Tag with the EC50 of 25. 7ng/ml determined by ELISA.

Human HGF R/c-MET protein (Recombinant) (C-His-Avi) {Biotin} (STJP001673)

SKU:
STJP001673

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human HGF R/c-MET-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: MET
Gene ID: 4233
Uniprot ID: MET_HUMAN
Immunogen Region: Glu25-Thr932
Immunogen: DNA sequence encoding Human HGF R/c-MET including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Autophosphorylated in response to ligand binding on Tyr-1234 and Tyr-1235 in the kinase domain leading to further phosphorylation of Tyr-1349 and Tyr-1356 in the C-terminal multifunctional docking site. Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. Dephosphorylated by PTPN1 and PTPN2. Ubiquitinated. Ubiquitination by CBL regulates MET endocytosis, resulting in decreasing plasma membrane receptor abundance, and in endosomal degradation and/or recycling of internalized receptors. O-mannosylation of IPT/TIG domains by TMEM260 is required for protein maturation. O-mannosylated residues are composed of single mannose glycans that are not elongated or modified. (Microbial infection) Tyrosine phosphorylation is stimulated by L.monocytogenes InlB. Tyrosine phosphorylation is maximal 10-20 minutes after treatment with InlB and disappears by 60 minutes. The phosphorylated residues were not identified.
Function Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis. (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.
Protein Name Hepatocyte Growth Factor Receptor
Hgf Receptor
Hgf/Sf Receptor
Proto-Oncogene C-Met
Scatter Factor Receptor
Sf Receptor
Tyrosine-Protein Kinase Met
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-416550
Reactome: R-HSA-5673001
Reactome: R-HSA-6806942
Reactome: R-HSA-6807004
Reactome: R-HSA-6811558
Reactome: R-HSA-8851805
Reactome: R-HSA-8851907
Reactome: R-HSA-8865999
Reactome: R-HSA-8874081
Reactome: R-HSA-8875360
Reactome: R-HSA-8875513
Reactome: R-HSA-8875555
Reactome: R-HSA-8875656
Reactome: R-HSA-8875791
Reactome: R-HSA-9022699
Reactome: R-HSA-9734091
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Isoform 3: Secreted
Alternative Protein Names Hepatocyte Growth Factor Receptor protein
Hgf Receptor protein
Hgf/Sf Receptor protein
Proto-Oncogene C-Met protein
Scatter Factor Receptor protein
Sf Receptor protein
Tyrosine-Protein Kinase Met protein
MET protein

Information sourced from Uniprot.org

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