Human HGF (HGF) Antibody Pair (STJAP00873)
General Information
| Short Description | This HGF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R03-4J-5/R02-4Z-9 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | HGF |
| Gene ID | 3082 |
| Uniprot ID | HGF_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Post Translational Modifications | The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form. The single-chain precursor undergoes proteolytic processing by HGFAC resulting in an active two-chain form. |
| Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation. |
| Protein Name | Hepatocyte Growth FactorHepatopoietin-AScatter FactorSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1257604Reactome: R-HSA-1266695Reactome: R-HSA-2219530Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-6806942Reactome: R-HSA-6807004Reactome: R-HSA-6811558Reactome: R-HSA-8851805Reactome: R-HSA-8851907Reactome: R-HSA-8865999Reactome: R-HSA-8874081Reactome: R-HSA-8875513Reactome: R-HSA-8875555Reactome: R-HSA-8875656Reactome: R-HSA-8875791Reactome: R-HSA-9734091 |
| Cellular Localisation | |
| Alternative Antibody Pair Names | Hepatocyte Growth Factor antibody pairHepatopoietin-A antibody pairScatter Factor antibody pairSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain antibody pairHGF antibody pairHPTA antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human HGF (HGF) Antibody Pair (STJAP00873)
This antibody pair is designed to detect human Hepatocyte Growth Factor (HGF), corresponding to UniProt accession P14210. Reactivity has been confirmed for human samples only.
Both antibodies in this pair are rabbit-derived monoclonal IgGs, with clone IDs R03-4J-5 and R02-4Z-9. Neither antibody carries a conjugate or tag, so end users are responsible for labeling the detection antibody according to their assay format.
This pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both antibodies were raised against a recombinant protein immunogen corresponding to human HGF. No specific immunogen region is defined in the current product specifications.
The antibodies should be stored at 4°C for short-term use or aliquoted and stored at -20°C for long-term storage. Repeated freeze/thaw cycles should be avoided to preserve activity.
The optimal working dilution for each antibody in this pair is not predefined and should be determined empirically by the end user. Titration experiments are recommended to establish the appropriate concentrations for the specific assay conditions being used.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.