Human Hepatocyte Growth Factor protein (Recombinant) (STJP000426)
SPECIFICATIONS
HostCHO Cells
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Human Hepatocyte Growth Factor mature chain was expressed in Chinese Hamster Ovary cell line. NA
General Information
| Short Description | Recombinant-Human Hepatocyte Growth Factor-protein was developed from cho cells. For use in research applications. |
| Host | CHO Cells |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered solution in 0.5% glycine, 0.5% sucrose,.01% Tween80, mM Glutamic acid, pH7.5. |
| Storage Instruction | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The ED (50) was determined by the dose-dependent stimulation of the proliferation ofmurine BNL-CL2cell li was found to be in the range of.0-40.0 ng/ml. NA |
Target Information
| Gene Symbol | HGF |
| Gene ID | 3082 |
| Uniprot ID | HGF_HUMAN |
| Immunogen | Optimized DNA sequence encoding Human Hepatocyte Growth Factor mature chain was expressed in Chinese Hamster Ovary cell line. NA |
| Immunogen Region | Mature chain |
| Immunogen Sequence | MWVTKLLPAL LLQHVLLHLL LLPIAIPYAE GQRKRRNTIH EFKKSAKTTL IKIDPALKIK TKKVNTADQC ANRCTRNKGL PFTCKAFVFD KARKQCLWFP FNSMSSGVKK EFGHEFDLYE NKDYIRNCII GKGRSYKGTV SITKSGIKCQ PWSSMIPHEH SFLPSSYRGK DLQENYCRNP RGEEGGPWCF TSNPEVRYEV CDIPQCSEVE CMTCNGESYR GLMDHTES |
Additional Info
| Post Translational Modifications | The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form. The single-chain precursor undergoes proteolytic processing by HGFAC resulting in an active two-chain form. |
| Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation. |
| Protein Name | Hepatocyte Growth FactorHepatopoietin-AScatter FactorSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1257604Reactome: R-HSA-1266695Reactome: R-HSA-2219530Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-6806942Reactome: R-HSA-6807004Reactome: R-HSA-6811558Reactome: R-HSA-8851805Reactome: R-HSA-8851907Reactome: R-HSA-8865999Reactome: R-HSA-8874081Reactome: R-HSA-8875513Reactome: R-HSA-8875555Reactome: R-HSA-8875656Reactome: R-HSA-8875791Reactome: R-HSA-9734091 |
| Cellular Localisation | |
| Alternative Protein Names | Hepatocyte Growth Factor proteinHepatopoietin-A proteinScatter Factor proteinSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain proteinHGF proteinHPTA protein |
Information sourced from Uniprot.org