Human GRP58/PDIA3 protein (Recombinant) (His-Tag) (STJP019200)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human GRP58/PDIA3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1 M NaCl, and 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity > 10 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT. |
Target Information
| Gene Symbol | PDIA3 |
| Gene ID | 2923 |
| Uniprot ID | PDIA3_HUMAN |
| Accession Number | NP_005304 |
| Immunogen | Human |
| Immunogen Region | 25-505aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Detected in the flagellum and head region of spermatozoa (at protein level). Expressed in liver, stomach and colon (at protein level). Expressed in gastric parietal cells and chief cells (at protein level). |
| Post Translational Modifications | Within the major histocompatibility complex class I (MHC I) peptide loading complex forms reversible disulfide-linked heterodimers with TAPBP as part of its protein folding chaperone activity. This is essential to assist the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Phosphorylated. |
| Function | Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone. Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity. |
| Protein Name | Protein Disulfide-Isomerase A358 Kda Glucose-Regulated Protein58 Kda Microsomal ProteinP58Disulfide Isomerase Er-60Endoplasmic Reticulum Resident Protein 57Er Protein 57Erp57Endoplasmic Reticulum Resident Protein 60Er Protein 60Erp60 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-901042Reactome: R-HSA-983170 |
| Cellular Localisation | Endoplasmic ReticulumEndoplasmic Reticulum LumenMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Protein Names | Protein Disulfide-Isomerase A3 protein58 Kda Glucose-Regulated Protein protein58 Kda Microsomal Protein proteinP58 proteinDisulfide Isomerase Er-60 proteinEndoplasmic Reticulum Resident Protein 57 proteinEr Protein 57 proteinErp57 proteinEndoplasmic Reticulum Resident Protein 60 proteinEr Protein 60 proteinErp60 proteinPDIA3 proteinERP57 proteinERP60 proteinGRP58 protein |
Information sourced from Uniprot.org