Human GRO Beta/CXCL2 (Growth Regulated Oncogene Beta) Sandwich ELISA Kit (STJE0003310)

SPECIFICATIONS
STJE0003310
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General Information

Short DescriptionThis GRO Beta/CXCL2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityHuman
Sensitivity9.38pg/mL
Detection Limit15.63~1000pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolCXCL2
Gene ID2920
Uniprot IDCXCL2_HUMAN
SpecificityThis kit recognizes Human GRO Beta/CXCL2 in samples. No significant cross-reactivity or interference between Human GRO Beta/CXCL2 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Function Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
Protein Name C-X-C Motif Chemokine 2
Growth-Regulated Protein Beta
Gro-Beta
Macrophage Inflammatory Protein 2-Alpha
Mip2-Alpha Cleaved Into - Gro-Beta(5-73
Gro-Beta-T
Hematopoietic Synergistic Factor
Hsf
Sb-251353
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-6783783
Cellular Localisation Secreted
Alternative ELISA Names C-X-C Motif Chemokine 2 ELISA kit
Growth-Regulated Protein Beta ELISA kit
Gro-Beta ELISA kit
Macrophage Inflammatory Protein 2-Alpha ELISA kit
Mip2-Alpha Cleaved Into - Gro-Beta(5-73 ELISA kit
Gro-Beta-T ELISA kit
Hematopoietic Synergistic Factor ELISA kit
Hsf ELISA kit
Sb-251353 ELISA kit
CXCL2 ELISA kit
GRO2 ELISA kit
GROB ELISA kit
MIP2A ELISA kit
SCYB2 ELISA kit
Specificity This kit recognizes Human GROβ/CXCL2 in samples. No significant cross-reactivity or interference between Human GROβ/CXCL2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-106 98
EDTA plasma (n=8) 91-104 97
Cell culture media (n=8) 86-97 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 54.70 135.50 382.40 59.70 146.50 360.80
Standard deviation 3.70 7.60 15.30 3.00 6.60 17.70
CV (%) 6.76 5.61 4.00 5.03 4.51 4.91

Citations

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