Human GREM1 (Gremlin-1) Sandwich ELISA Kit (STJE0002902)

Name: Human GREM1 (Gremlin-1) Sandwich ELISA Kit (STJE0002902)
SKU: STJE0002902
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002902

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Short Description	This GREM1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	GREM1
Gene ID	26585
Uniprot ID	GREM1_HUMAN
Specificity	This kit recognizes Human GREM1 in samples. No significant cross-reactivity or interference between Human GREM1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Antagonist of BMP2.inhibits BMP2-mediated differentiation of osteoblasts (in vitro). Acts as inhibitor of monocyte chemotaxis. Can inhibit the growth or viability of normal cells but not transformed cells when is overexpressed.
Protein Name	Gremlin-1 Cell Proliferation-Inducing Gene 2 Protein Cysteine Knot Superfamily 1 - Bmp Antagonist 1 Dan Domain Family Member 2 Down-Regulated In Mos-Transformed Cells Protein Increased In High Glucose Protein 2 Ihg-2
Database Links	Reactome: R-HSA-9830674
Cellular Localisation	Secreted
Alternative ELISA Names	Gremlin-1 ELISA kit Cell Proliferation-Inducing Gene 2 Protein ELISA kit Cysteine Knot Superfamily 1 - Bmp Antagonist 1 ELISA kit Dan Domain Family Member 2 ELISA kit Down-Regulated In Mos-Transformed Cells Protein ELISA kit Increased In High Glucose Protein 2 ELISA kit Ihg-2 ELISA kit GREM1 ELISA kit CKTSF1B1 ELISA kit DAND2 ELISA kit DRM ELISA kit PIG2 ELISA kit
Specificity	This kit recognizes Human GREM1 in samples. No significant cross-reactivity or interference between Human GREM1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.47	1.43	4.34	0.45	1.38	4.48
Standard deviation	0.03	0.06	0.13	0.03	0.08	0.24
CV (%)	6.38	4.20	3.00	6.67	5.80	5.36