Human GLS (Glutaminase) Sandwich ELISA Kit Ready-To-Use Kit (STJE0015583)
SPECIFICATIONS
General Information
| Short Description | This GLS Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.117ng/mL |
| Detection Limit | 0.312-20ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | GLS |
| Gene ID | 2744 |
| Uniprot ID | GLSK_HUMAN |
| Immunogen Region | Ready-To-Use |
| Sample Type | serum, plasma, tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle. |
| Post Translational Modifications | Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits. |
| Function | Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain. Isoform 2: Lacks catalytic activity. |
| Protein Name | Glutaminase Kidney Isoform - MitochondrialGlsK-GlutaminaseL-Glutamine Amidohydrolase Cleaved Into - Glutaminase Kidney Isoform - Mitochondrial 68 Kda Chain - Glutaminase Kidney Isoform - Mitochondrial 65 Kda Chain |
| Database Links | Reactome: R-HSA-210500Reactome: R-HSA-5628897Reactome: R-HSA-8964539 |
| Cellular Localisation | Isoform 1: MitochondrionCytoplasmCytosolThe 74-Kda Cytosolic Precursor Is Translocated Into The Mitochondria And Processed Via A 72-Kda Intermediate To Yield The Mature 68- And 65-Kda SubunitsIsoform 3: MitochondrionGlutaminase Kidney IsoformMitochondrial 68 Kda Chain: Mitochondrion MatrixProduced By The Proteolytic Processing Of The 74-Kda Cytosolic PrecursorMitochondrial 65 Kda Chain: Mitochondrion Matrix |
| Alternative ELISA Names | Glutaminase Kidney Isoform - Mitochondrial ELISA kitGls ELISA kitK-Glutaminase ELISA kitL-Glutamine Amidohydrolase Cleaved Into - Glutaminase Kidney Isoform - Mitochondrial 68 Kda Chain - Glutaminase Kidney Isoform - Mitochondrial 65 Kda Chain ELISA kitGLS ELISA kitGLS1 ELISA kitKIAA0838 ELISA kit |
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Information sourced from Uniprot.org