| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Recombinant-Human Glial growth factor (GGF2)-protein was developed from hek293. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered 0.02M PB, pH7.0. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The ED (50) was determined by the dose-dependentphosphorylation of human MCF-7 cells was found to be <20ng/ml, corresponding to a specific activity ofx Units/mg. NA |
| Gene Symbol: | NRG1 |
| Gene ID: | 3084 |
| Uniprot ID: | NRG1_HUMAN |
| Immunogen Region: | Isoform 9 |
| Immunogen: | Optimized DNA sequence encoding Human Neuregulin-1 isoform 9, GGF2 was expressed in HEK cells. |
| Tissue Specificity | Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells.in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. |
| Post Translational Modifications | Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage. |
| Function | Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells.inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction.stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells.stimulating Schwann cell proliferation.implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4. Binds to ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons. |
| Protein Name | Pro-Neuregulin-1 - Membrane-Bound IsoformPro-Nrg1 Cleaved Into - Neuregulin-1Acetylcholine Receptor-Inducing ActivityAriaBreast Cancer Cell Differentiation Factor P45Glial Growth FactorHeregulinHrgNeu Differentiation FactorSensory And Motor Neuron-Derived Factor |
| Database Links | Reactome: R-HSA-1227986 Q02297-10Reactome: R-HSA-1236394 Q02297-10Reactome: R-HSA-1250196 Q02297-10Reactome: R-HSA-1250342Reactome: R-HSA-1250347Reactome: R-HSA-1251985Reactome: R-HSA-1257604 Q02297-10Reactome: R-HSA-1306955 Q02297-10Reactome: R-HSA-1358803 Q02297-10Reactome: R-HSA-1963640Reactome: R-HSA-1963642 Q02297-10Reactome: R-HSA-2219530 Q02297-10Reactome: R-HSA-5673001 Q02297-10Reactome: R-HSA-6785631 Q02297-10Reactome: R-HSA-6811558 Q02297-10Reactome: R-HSA-8847993 Q02297-10Reactome: R-HSA-8863795 Q02297-10Reactome: R-HSA-9620244Reactome: R-HSA-9664565 Q02297-10Reactome: R-HSA-9665686 Q02297-10 |
| Cellular Localisation | Pro-Neuregulin-1Membrane-Bound Isoform: Cell MembraneSingle-Pass Type I Membrane ProteinDoes Not Seem To Be ActiveNeuregulin-1: SecretedIsoform 8: NucleusMay Be NuclearIsoform 9: SecretedHas A Signal PeptideIsoform 10: MembraneMay Possess An Internal Uncleaved Signal Sequence |
| Alternative Protein Names | Pro-Neuregulin-1 - Membrane-Bound Isoform proteinPro-Nrg1 Cleaved Into - Neuregulin-1 proteinAcetylcholine Receptor-Inducing Activity proteinAria proteinBreast Cancer Cell Differentiation Factor P45 proteinGlial Growth Factor proteinHeregulin proteinHrg proteinNeu Differentiation Factor proteinSensory And Motor Neuron-Derived Factor proteinNRG1 proteinGGF proteinHGL proteinHRGA proteinNDF proteinSMDF protein |
Information sourced from Uniprot.org
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