This GLI1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids..
Applications
ELISA
Reactivity
Human
Sensitivity
0.122ng/mL
Detection Limit
0.312-20ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
tissue homogenates, cell lysates or other biological fluids.
Additional Info
Tissue Specificity
Detected in testis (at protein level). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta.isoform 2 is not detectable in these tissues.
Post Translational Modifications
Phosphorylated in vitro by ULK3. Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1. Ubiquitinated by the CRL2(FEM1B) complex, suppressing GLI1 transcriptional activator activity.
Function
Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling. Isoform 2: Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.
Protein Name
Zinc Finger Protein Gli1Glioma-Associated OncogeneOncogene Gli
CytoplasmNucleusTethered In The Cytoplasm By Binding To SufuActivation And Translocation To The Nucleus Is Promoted By Interaction With Stk36Phosphorylation By Ulk3 May Promote Nuclear LocalizationTranslocation To The Nucleus Is Promoted By Interaction With Zic1Isoform 2: Cytoplasm
Alternative ELISA Names
Zinc Finger Protein Gli1 ELISA kitGlioma-Associated Oncogene ELISA kitOncogene Gli ELISA kitGLI1 ELISA kitGLI ELISA kit
