Human GET4 protein (Recombinant) (His-Tag) (STJP018658)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human GET4-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 0.4M urea |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GET4 |
| Gene ID | 51608 |
| Uniprot ID | GET4_HUMAN |
| Accession Number | NP_057033 |
| Immunogen | Human |
| Immunogen Region | 1-327aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSMAAAAAM AEQESARNGG RNRGGVQRVE GKLRASVEKG DYYEAHQMYR TLFFRYMSQS KHTEARELMY SGALLFFSHG QQNSAADLSM LVLESLEKAE VEVADELLEN LAKVFSLMDP NSPERVTFVS RALKWSSGGS GKLGHPRLHQ LLALTLWKEQ NYCESRYHFL HSADGEGCAN MLVEYSTSRG FRSEVDMFVA QAVLQFLC |
Additional Info
| Post Translational Modifications | Ubiquitinated by RNF12, leading to proteasomal degradation. When unassembled from BAG6.ubiquitinylation is modulated by BAG6 quality control role and effectuated by RNF126. |
| Function | As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates in their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation. The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum. Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome. Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome. |
| Protein Name | Golgi To Er Traffic Protein 4 HomologConserved Edge-Expressed ProteinTransmembrane Domain Recognition Complex 35 Kda SubunitTrc35 |
| Database Links | Reactome: R-HSA-9609523 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Protein Names | Golgi To Er Traffic Protein 4 Homolog proteinConserved Edge-Expressed Protein proteinTransmembrane Domain Recognition Complex 35 Kda Subunit proteinTrc35 proteinGET4 proteinC7orf20 proteinCEE proteinTRC35 proteinCGI-20 protein |
Information sourced from Uniprot.org