Human GDNFR-alpha-2/GFRA2 protein (Recombinant) (C-His) (STJP000972)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human GDNFR-alpha-2/GFRA2 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser22-Ser441) of human GFRA2/GFR Alpha 2/GDNFRB (Accession #NP_001486.4) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human GDNFR-alpha-2/GFRA2-C-His protein was developed in hek293 cells using the region Ser22-Ser441. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized recombinant Human Neurturin at 5 Mu g/mL (100 Mu L/well) can bind recombinant Human GFRA2, the EC50 of Human GFRA2 is 1.82 Mu g/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | GFRA2 |
| Gene ID | 2675 |
| Uniprot ID | GFRA2_HUMAN |
| Immunogen | Recombinant Human GDNFR-alpha-2/GFRA2 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser22-Ser441) of human GFRA2/GFR Alpha 2/GDNFRB (Accession #NP_001486.4) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Ser22-Ser441 |
| Immunogen Sequence | SPSSLQGPELHGWRPPVDCV RANELCAAESNCSSRYRTLR QCLAGRDRNTMLANKECQAA LEVLQESPLYDCRCKRGMKK ELQCLQIYWSIHLGLTEGEE FYEASPYEPVTSRLSDIFRL ASIFSGTGADPVVSAKSNHC LDAAKACNLNDNCKKLRSSY ISICNREISPTERCNRRKCH KALRQFFDRVPSEYTYRMLF CSCQDQACAERRRQTILPSC SYEDKEKPNCLDLRGVCRT |
Additional Info
| Tissue Specificity | Isoform 1: found in both brain and placenta. |
| Function | Receptor for neurturin (NRTN), a growth factor that supports the survival of sympathetic neurons. NRTN-binding leads to autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. Isoform 2: Participates in NRTN-induced 'Ser-727' phosphorylation of STAT3. |
| Protein Name | Gdnf Family Receptor Alpha-2Gdnf Receptor Alpha-2Gdnfr-Alpha-2Gfr-Alpha-2Gdnf Receptor BetaGdnfr-BetaNeurturin Receptor AlphaNrtnr-AlphaNtnr-AlphaRet Ligand 2Tgf-Beta-Related Neurotrophic Factor Receptor 2 |
| Database Links | Reactome: R-HSA-419037Reactome: R-HSA-5673001Reactome: R-HSA-8853659 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative Protein Names | Gdnf Family Receptor Alpha-2 proteinGdnf Receptor Alpha-2 proteinGdnfr-Alpha-2 proteinGfr-Alpha-2 proteinGdnf Receptor Beta proteinGdnfr-Beta proteinNeurturin Receptor Alpha proteinNrtnr-Alpha proteinNtnr-Alpha proteinRet Ligand 2 proteinTgf-Beta-Related Neurotrophic Factor Receptor 2 proteinGFRA2 proteinGDNFRB proteinRETL2 proteinTRNR2 protein |
Information sourced from Uniprot.org