Human GDF9 (Growth/differentiation factor 9) Sandwich ELISA Kit (STJE0003319)

Name: Human GDF9 (Growth/differentiation factor 9) Sandwich ELISA Kit (STJE0003319)
SKU: STJE0003319
Availability: InStock

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SPECIFICATIONS

STJE0003319

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Short Description	This GDF9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	GDF9
Gene ID	2661
Uniprot ID	GDF9_HUMAN
Specificity	This kit recognizes Human GDF9 in samples. No significant cross-reactivity or interference between Human GDF9 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylated.phosphorylation is critical for GDF9 function. In vitro, can be phosphorylated by CK at Ser-325.
Function	Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.
Protein Name	Growth/Differentiation Factor 9 Gdf-9
Cellular Localisation	Secreted
Alternative ELISA Names	Growth/Differentiation Factor 9 ELISA kit Gdf-9 ELISA kit GDF9 ELISA kit
Specificity	This kit recognizes Human GDF9 in samples. No significant cross-reactivity or interference between Human GDF9 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.50	0.84	4.97	0.51	0.82	4.85
Standard deviation	0.03	0.04	0.23	0.03	0.04	0.15
CV (%)	6.00	4.76	4.63	5.88	4.88	3.09