Human Galectin-3/LGALS3 protein (Recombinant) (No tag) (STJP000832)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Human Galectin-3/LGALS3 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala2-Ile250) of human Galectin-3 (Accession #NP_002297.2).
General Information
| Short Description | Recombinant-Human Galectin-3/LGALS3-No tag protein was developed in e.coli using the region Ala2-Ile250. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human Galectin-3 at 1 Mu g/mL (100 Mu L/well) can bind Galectin-3/LGALS3 Rabbit mAb with a linear range of 0.24-35 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | LGALS3 |
| Gene ID | 3958 |
| Uniprot ID | LEG3_HUMAN |
| Immunogen | Recombinant Human Galectin-3/LGALS3 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala2-Ile250) of human Galectin-3 (Accession #NP_002297.2). |
| Immunogen Region | Ala2-Ile250 |
| Immunogen Sequence | ADNFSLHDALSGSGNPNPQG WPGAWGNQPAGAGGYPGASY PGAYPGQAPPGAYPGQAPPG AYPGAPGAYPGAPAPGVYPG PPSGPGAYPSSGQPSATGAY PATGPYGAPAGPLIVPYNLP LPGGVVPRMLITILGTVKPN ANRIALDFQRGNDVAFHFNP RFNENNRRVIVCNTKLDNNW GREERQSVFPFESGKPFKIQ VLVEPDHFKVAVNDAHLLQY NHRVKKLNEISKLGISGDI |
Additional Info
| Tissue Specificity | A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. |
| Function | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes. |
| Protein Name | Galectin-3Gal-335 Kda LectinCarbohydrate-Binding Protein 35Cbp 35Galactose-Specific Lectin 3Galactoside-Binding ProteinGalbpIge-Binding ProteinL-31Laminin-Binding ProteinLectin L-29Mac-2 Antigen |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-879415Reactome: R-HSA-8939246Reactome: R-HSA-8941333 |
| Cellular Localisation | CytoplasmNucleusSecretedSecreted By A Non-Classical Secretory Pathway And Associates With The Cell SurfaceCan Be SecretedThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Protein Names | Galectin-3 proteinGal-3 protein35 Kda Lectin proteinCarbohydrate-Binding Protein 35 proteinCbp 35 proteinGalactose-Specific Lectin 3 proteinGalactoside-Binding Protein proteinGalbp proteinIge-Binding Protein proteinL-31 proteinLaminin-Binding Protein proteinLectin L-29 proteinMac-2 Antigen proteinLGALS3 proteinMAC2 protein |
Information sourced from Uniprot.org