Human Galectin-3/LGALS3 protein (Recombinant) (His-Tag) (STJP016556)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP016556
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General Information

Short DescriptionRecombinant-Human Galectin-3/LGALS3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE/Bioactivity
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 1mM DTT, 0.1M NaCl
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
ImmunoreactivityThe ED50 for this effect is less or equal to 15ug/ml. Measured by its ability to agglutinate human red blood cells.

Target Information

Gene SymbolLGALS3
Gene ID3958
Uniprot IDLEG3_HUMAN
Accession NumberNP_002297.2
ImmunogenHuman
Immunogen Region1-250aa
Immunogen Sequence MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEP

Additional Info

Tissue Specificity A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.
Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
Protein Name Galectin-3
Gal-3
35 Kda Lectin
Carbohydrate-Binding Protein 35
Cbp 35
Galactose-Specific Lectin 3
Galactoside-Binding Protein
Galbp
Ige-Binding Protein
L-31
Laminin-Binding Protein
Lectin L-29
Mac-2 Antigen
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-879415
Reactome: R-HSA-8939246
Reactome: R-HSA-8941333
Cellular Localisation Cytoplasm
Nucleus
Secreted
Secreted By A Non-Classical Secretory Pathway And Associates With The Cell Surface
Can Be Secreted
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative Protein Names Galectin-3 protein
Gal-3 protein
35 Kda Lectin protein
Carbohydrate-Binding Protein 35 protein
Cbp 35 protein
Galactose-Specific Lectin 3 protein
Galactoside-Binding Protein protein
Galbp protein
Ige-Binding Protein protein
L-31 protein
Laminin-Binding Protein protein
Lectin L-29 protein
Mac-2 Antigen protein
LGALS3 protein
MAC2 protein

Information sourced from Uniprot.org

Citations

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