Human GAD1 (Glutamate decarboxylase 1) CLIA Kit for Quantitative Detection

Name: Human GAD1 (Glutamate decarboxylase 1) CLIA Kit for Quantitative Detection
SKU: STJC0000970
Availability: InStock

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SPECIFICATIONS

STJC0000970

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Short Description	This human GAD1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	0.19ng/mL
Detection Limit	0.31~20ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	GAD1
Gene ID	2571
Uniprot ID	DCE1_HUMAN
Specificity	This kit recognizes Human GAD1 in samples. No significant cross-reactivity or interference between Human GAD1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Isoform 1: Expressed in brain. Isoform 3: Expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
Function	Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor. Isoform 3: Enzymatically inactive as glutamate decarboxylase.
Protein Name	Glutamate Decarboxylase 1 67 Kda Glutamic Acid Decarboxylase Gad-67 Glutamate Decarboxylase 67 Kda Isoform
Database Links	Reactome: R-HSA-888568 Reactome: R-HSA-888590 Reactome: R-HSA-9022927
Cellular Localisation
Alternative CLIA Names	Glutamate Decarboxylase 1 CLIA kit 67 Kda Glutamic Acid Decarboxylase CLIA kit Gad-67 CLIA kit Glutamate Decarboxylase 67 Kda Isoform CLIA kit GAD1 CLIA kit GAD CLIA kit GAD67 CLIA kit
Specificity	This kit recognizes Human GAD1 in samples. No significant cross-reactivity or interference between Human GAD1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.99	2.40	9.36	1.01	2.23	10.04
Standard deviation	0.12	0.28	0.90	0.10	0.16	1.13
CV (%)	12.12	11.67	9.62	9.90	7.17	11.25