Human GABARAP protein (Recombinant) (His-Tag) (STJP016607)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human GABARAP-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 20% Glycerol 0.2M NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GABARAP |
| Gene ID | 11337 |
| Uniprot ID | GBRAP_HUMAN |
| Accession Number | NP_009209 |
| Immunogen | Human |
| Immunogen Region | 1-117aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL |
Additional Info
| Tissue Specificity | Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, GABARAP-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, GABARAP-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). |
| Function | Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction. Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation. Involved in apoptosis. |
| Protein Name | Gamma-Aminobutyric Acid Receptor-Associated ProteinGaba(A Receptor-Associated ProteinMm46 |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-8854214 |
| Cellular Localisation | Cytoplasmic VesicleAutophagosome MembraneEndomembrane SystemCytoplasmCytoskeletonGolgi Apparatus MembraneLargely Associated With Intracellular Membrane Structures Including The Golgi Apparatus And Postsynaptic CisternaeColocalizes With MicrotubulesAlso Localizes To Discrete Punctae Along The Ciliary Axoneme |
| Alternative Protein Names | Gamma-Aminobutyric Acid Receptor-Associated Protein proteinGaba(A Receptor-Associated Protein proteinMm46 proteinGABARAP proteinFLC3B proteinHT004 protein |
Information sourced from Uniprot.org