Human G6PD (Glucose-6-phosphate 1-dehydrogenase) CLIA Kit for Quantitative Detection

Name: Human G6PD (Glucose-6-phosphate 1-dehydrogenase) CLIA Kit for Quantitative Detection
SKU: STJC0000794
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJC0000794

Current Stock:

🚚 Free UK Delivery on orders over £150

Quantity:

Processing The item has been added

Enquire For Bulk Order

✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

Short Description	This human G6PD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	37.5pg/mL
Detection Limit	62.5~4000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	G6PD
Gene ID	2539
Uniprot ID	G6PD_HUMAN
Specificity	This kit recognizes Human G6PD in samples. No significant cross-reactivity or interference between Human G6PD and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Isoform Long is found in lymphoblasts, granulocytes and sperm.
Post Translational Modifications	Acetylated by ELP3 at Lys-403.acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403.deacetylation stimulates its enzyme activity.
Function	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Protein Name	Glucose-6-Phosphate 1-Dehydrogenase G6pd
Database Links	Reactome: R-HSA-5628897 Reactome: R-HSA-71336 Reactome: R-HSA-9818028
Cellular Localisation	Cytoplasm Cytosol Membrane Peripheral Membrane Protein
Alternative CLIA Names	Glucose-6-Phosphate 1-Dehydrogenase CLIA kit G6pd CLIA kit G6PD CLIA kit
Specificity	This kit recognizes Human G6PD in samples. No significant cross-reactivity or interference between Human G6PD and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	206.49	361.06	1587.89	209.52	373.10	1659.47
Standard deviation	20.19	38.53	140.69	26.97	32.68	124.13
CV (%)	9.78	10.67	8.86	12.87	8.76	7.48