Human G3BP1 protein (Recombinant) (His-Tag) (STJP016979)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human G3BP1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 2mM DTT, 100mM NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | G3BP1 |
| Gene ID | 10146 |
| Uniprot ID | G3BP1_HUMAN |
| Accession Number | NP_005745.1 |
| Immunogen | Human |
| Immunogen Region | 1-466aa |
| Immunogen Sequence | MVMEKPSPLL VGREFVRQYY TLLNQAPDML HRFYGKNSSY VHGGLDSNGK PADAVYGQKE IHRKVMSQNF TNCHTKIRHV DAHATLNDGV VVQVMGLLSN NNQALRRFMQ TFVLAPEGSV ANKFYVHNDI FRYQDEVFGG FVTEPQEESE EEVEEPEERQ QTPEVVPDDS GTFYDQAVVS NDMEEHLEEP VAEPEPDPEP EPEQEPVSEI QEEKPEPVLE ETAPEDAQ |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylation of the acidic disordered region regulates stress granule assembly. RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization. Ubiquitinated by TRIM21 via 'Lys-63'-linked polyubiquitination in the NTF2 domain in response to heat shock, leading to stress granule disassembly: ubiquitination promotes interaction with the FAF2 adapter, followed by interaction with VCP, which extracts G3BP1 from stress granules, leading to stress granule disassembly. In case of prolonged stress, ubiquitination by TRIM21 leads to autophagy-dependent degradation of G3BP1 via recruitment of ubiquitinated G3BP1 by SQSTM1 and/or CALCOCO2 to autophagosomes. (Microbial infection) Cleaved by human enterovirus 71.this cleavage induces the disassembly of cytoplasmic stress granules. Cleaved by Foot-and-mouth disease virus.this cleavage suppresses the formation of cytoplasmic stress granules. Arg-435 is dimethylated, probably to asymmetric dimethylarginine. (Microbial infection) Cleaved by Encephalomyocarditis virus protease 3C.this cleavage suppresses the formation of cytoplasmic stress granules. |
| Function | Protein involved in various processes, such as stress granule formation and innate immunity. Plays an essential role in stress granule formation. Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress. Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations. Also acts as an ATP- and magnesium-dependent helicase: unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Plays an essential role in innate immunity by promoting CGAS and RIGI activity. Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS. Triggers the condensation of cGAS, a process probably linked to the formation of membrane-less organelles. Also enhances RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA. May also act as a phosphorylation-dependent sequence-specific endoribonuclease in vitro: Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. |
| Protein Name | Ras Gtpase-Activating Protein-Binding Protein 1G3bp-1Atp-Dependent Dna Helicase ViiiHdh ViiiGap Sh3 Domain-Binding Protein 1 |
| Database Links | Reactome: R-HSA-9705671 |
| Cellular Localisation | CytoplasmCytosolPerikaryonStress GranuleNucleusCytoplasmic In Proliferating CellsCytosolic And Partially Nuclear In Resting CellsRecruited To Stress Granules In Response To Arsenite TreatmentThe Unphosphorylated Form Is Recruited To Stress GranulesHras Signaling Contributes To This Process By Regulating G3bp Dephosphorylation |
| Alternative Protein Names | Ras Gtpase-Activating Protein-Binding Protein 1 proteinG3bp-1 proteinAtp-Dependent Dna Helicase Viii proteinHdh Viii proteinGap Sh3 Domain-Binding Protein 1 proteinG3BP1 proteinG3BP protein |
Information sourced from Uniprot.org